High-performance thin-layer chromatography fingerprinting and antioxidant study of Mangifera indica mother tincture

Authors

Binit Kumar Dwivedi, Dr D P Rastogi Central Research Institute of Homoeopathy, Noida, Uttar Pradesh, IndiaFollow
Manoj Kumar, Dr D P Rastogi Central Research Institute of Homoeopathy, Noida, Uttar Pradesh, IndiaFollow
Rakhi Mishra, Dr D P Rastogi Central Research Institute of Homoeopathy, Noida, Uttar Pradesh, IndiaFollow

Corresponding Author

Binit Kumar Dwivedi

Keywords

Physicochemical, Phytochemical, Mangifera indica, High performance thin layer chromatography, U.V spectroscopy.

Article Type

Original Article

Abstract

Background Mangifera indica is widely used in the Homoeopathy systems of medicine. In Homoeopathy, Mang. ind. is one of the indicated medicines for passive haemorrhages, uterine, renal and gastric problems. Mangiferin is a xanthanoid present in leaves, stalks barks, peel and kernel of the mango plant. Mangiferin has antioxidant, antiviral, anticancer, antidiabetic, antiaging, immunomodulatory, hepatoprotective and analgesic properties. Therefore in the current study, we have tried to identify mangiferin and its antioxidant properties in Mang. ind. homoeopathic mother tincture. Objective The aim of the present study wais to identify Mangiferin, Total Phenol Content (TPC) and Total Flavonoid Content (TFC) in Mang. ind. homoeopathic mother tincture. Materials and Methods Mangiferin (A) was used as a primary reference standard and in sample In-house prepared mother tincture (B) and two different market samples (C and D) were used. An authentic sample of Mang. ind. bark was received from the Centre of Medicinal Plants Research in Homoeopathy, Emerald, Tamil Nadu, India for preparation of in house mother tincture. Results We have successfully identified Mangiferin in Mang. ind. in-house prepared mother tincture (B) and two market samples (C and D) by HPTLC. Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) was determined by Ultraviolet–visible spectroscopy. Conclusion High Performance Thin Layer Chromatography study confirms the presence of Mangiferin whereas U. V spectroscopy proves to be a useful tool for in-vitro antioxidant assay study.

Digital Object Identifier

10.53945/2320-7094.1033

Publisher

Central Council for Research in Homoeopathy

How to cite this article

Dwivedi BK, Kumar M, Mishra R. High-performance thin-layer chromatography fingerprinting and antioxidant study of Mangifera indica mother tincture. Indian J Res Homoeopathy 2021;15. doi: 10.53945/2320-7094.1033